Business in Plant Tissue Culture

Improving Plants through Tissue Culture

More than micro-propagation, the field of controlled management and breeding of plant germplasm at the cellular level has received more impetus from this technology. Plant tissue culture techniques have greatly enhanced the ability of today’s industry to break, arrange and reorganize the constituents of higher plants. The commercial viability of this technique has been demonstrated in case of a number of model systems evolved today. However, a great deal of basic research has been already carried out on ornamental trees and shrubs with a view to develop new varieties of better landscape plants.

Selection of Plants with Better Capabilities of Stress and Pest Resistance

In fact, the most widely researched segment of tissue culture today is the idea of selecting advantageous varieties of plants that possess better capabilities of stress, disease and pest resistance. While the process of selecting and developing superior individuals has resulted in remarkable gains in the adaptability of a number of plant species, the search for superior individuals is accelerated, made easier and highly dependable by vitro systems. These systems endeavor to exploit the phenomenon of natural variability observed in plants. Alternatively, variability in plants can also be induced by chemical or physical agents that cause mutations in plants.

Plant tissues grown in vitro can be freed from the whole plant through the process of callus formation. If the resulting cells are exposed to a stress or susceptibility condition, only the tolerant ones survive while the susceptible ones are killed. This idea can be applied to a number of stress conditions as well as to achieve resistance to fungal and bacterial pathogens and phyto-toxic chemical agents. The process of selecting resistant cell lines help in reorganizing whole plants that can retain the resistance criteria selected. Some of the major achievements in this arena include selection of salt tolerant varieties of tomato, evolving tobacco plants that are resistant to freezing conditions and developing agronomic crops that are resistant to herbicides besides cultivating various plant species with improved resistance to pathogens.

Tissue Culture to Evolve Pathogen-free Plants

Plant tissue culture is the ideally suited technique to obtain, maintain and propagate pathogen-free plants in a commercial scale. The conventional system of labeling pest-free plants is akin to the idea of applying tissue culture as a selection process. Under this process, plants tested and reported free of pathogens like virus, bacteria and fungi are manually selected as explants for tissue culture. Most commonly, the apical domes of fast growing shoot tips are selected for this process and are grown under sterile conditions in vitro culture. The plantlets that result in the process are tested for the presence of pathogens and indexing is done. Those cultures indexed to be free of pathogens are maintained as explants stock.

Somatic Hybridization

Somatic Hybridization refers to the process of fusing plant cells gathered from different plant species that are incompatible for sexual crosses. Somatic Hybridization achieved through plant tissue culture makes it possible for plants to take in and incorporate in them foreign genetic codes in order to significantly extend the realms of plant modification. Somatic Hybridization employing plant tissue culture technique makes use of plant protoplasts. Protoplasts can be understood as single cells that are stripped of their cell walls by enzymatic treatment. A single leaf of a plant treated in this manner can yield several millions of single cells with each one of them capable of producing a whole plant eventually. The main concept underlying this technique is that plant cells stripped off their walls and brought into close contact tend to easily fuse with each other. Therefore, this method can overcome the limitations in plant breeding where it is rather impossible to hybridize species that are incompatible with each other. Though the concept is yet to be proved practically, the theory means that there is an infinite scope to evolve innumerable varieties of plants using this method.

20 thoughts on “Business in Plant Tissue Culture”

  1. Dear Sir,

    I have done Bsc Biotechnology and MBA in Marketinng and willing to start plant tissue culture firm.
    You have provided great information.I will apperciate if you can send some more information as to set up plant tissue culture business like from where I can raise funds? also if any references you have please help me.

  2. @Vrushali
    I am Dr Manisha Acharya,Manager KIIT-Technology Business Incubator (KIIT-TBI) KIIT University, Bhubaneswar,Orissa,India .
    We are the facilitators for incubating ideas and innovation having commercial potential in Biotech and ICT area.
    We can provide infrastructure requirements including seed funding for initial development of business.
    If you are interested to develop a Plant tissue culture firm at Bhubaneswar,Orissa you can contact to my email, my email id is

    1. Madhusudan Agrawal

      Hello Ma’m
      I am Post Graduate in Biotechnology and want to start a plant tissue culture lab in Orissa, i dont have fund to start. Can you give me details, how can i raise funds. And is it necessary to start the lab in BBSR, cant i start it in my hometown of Bhawanipatna, Dist. Kalahandi.

      Madhusudan Agrawal

  3. Dear All,

    We are plant tissue culture lab based in hyderabad. Certified by Dept of Biotechnology,India,produce 8 million Tc banana plants. We are looking for growers in UP,MP,Chattisgarh and West Bengal who would be interested in planting banana plants.Please mail us @

  4. Irfan urrahman

    I am a agriculture graduate want to start a small scale agri bussines,if anybody have suggestions please mail me.

  5. vivek chakravarti

    i had complete my bsc biotech. noe i doing msc. i want to information for business of plant tissue culture. plz give me information

  6. hello sir i m student of MSc in plant biotechnology and i want to start a small business in plant tissue culture, so please give me some ideas how to start the business

  7. ear sir,
    am a post graduate in biotech, with more than 4 yrs experience in bio fertilizers field, now working as entomologist in Dubai. i am in search for a potent business idea in biotech realm to start in India. how to start a PTC business, or any other idea you can give me? expecting a prompt reply,
    thanks and regards
    Sarath raj

    1. hi sir, i am into PTC since 6 yrs,if u are interested in this business contact me.
      expecting a reply soon,
      thanks and regards

  8. Madhusudan Agrawal

    Hello Sir,
    I have completed my MSc. in Biotech and looking to start a new business related to biotech, more eager to do it in plant tissue culture but want to know the prospects and scope of tissue culture in orissa. Please mail me on

    Madhusudan Agrawal


    I wish to start ptc buissnes. for this i required a project report & information about market potential of ptc.

  10. Hello sir, I had completed my bsc biotechnology and now I want to work in plant tissue culture area….. plz guide me. Is there any kind of opportunity in Bihar and Jharkhand.. plz do rply by mail .. Thank you

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